Using the Yeast2Hybrid system, the binding affinities between Ufd2a variants and VCP/p97 mutants were analyzed. Several VCP/p97 mutants are associated with Inclusion body myositis in association of Paget's disease of the bone and fronto temperal dementia (IMBPFD). It was found that Ufd2a bound strongest to the VCP disease causing mutant A232E. It was also determined that the muscle specific isoform of Ufd2a, Ufd2a III, did not interact with VCP/p97.
St. Germain, Amanda, "Ufd2a Isoforms with VSP/p97 and its IBMPFD Mutants" (2011). Honors Projects Overview. 51.
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