Document Type
Honors
Department
Biology
Rights Management
Rhode Island College
Abstract
The goal of this experiment was to determine if lower temperatures could serve to induce runaway replication for the expression of the lacZ gene in Vibrio species B-18 carrying a different runaway replication vector, pJM9487. One experiment compares β-galactosidase activity in cells incubated at 35, 37, and 39°C while the second experiment examines the enzyme levels for cells incubated at either a low temperature or a high temperature in the presence and absence of the chemical inducer used for the promoter (ptrp) that controls the expression of the lacZ gene on this plasmid.
Recommended Citation
Ramirez, Roberto, "Examining Escherichia Coli Plasmid Gene Expression in Vibrio Species B-18" (2014). Honors Projects. 103.
https://digitalcommons.ric.edu/honors_projects/103
Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-No Derivative Works 4.0 International License.